Faculdade de Ciências e Tecnologia
Departamento de Química
The course aims at providing an integrated overview on Enzyme Technology and the applications at industrial, analytical and healthcare levels. The basic concepts of enzymology and biocatalysts will be discussed with a particular emphasis on the activity and stability of the biocatalyst. The students should acquire knowledge to understand the processes of selection, production and purification of a biocatalyst as well as its application and modelling in bioreactors, namely through immobilization techniques.
(i) Enzyme structure and function.
(ii) Activity and stability of enzymes. Enzyme kinetics, effect of inhibitors on enzyme kinetics, enzyme denaturation and deactivation.
(iii) Enzyme production and purification.Sources of biocatalysts. Production of biocatalysts by fermentation. Methods for enzyme recovery: solid-liquid separation, cell lysis and disruption, protein precipitation, aqueous two-phase liquid extraction, chromatography.
(iv) Methods to increase the activity and stability of biocatalysts.Protein engineering, molecular modelling. Immobilization of biocatalysts.
(v) Characterization of immobilised biocatalysts. Effects of immobilization on enzyme kinetics, internal and external diffusional limitations.
(vi) Reactors with biocatalysts (soluble and immobilized biocatalysts), project and analysis. Continuous stirred tank reactor, plug flow reactor, batch reactor.
 Klaus Buchholz, Volker Kasche, Uwe Theo Bornscheuer (2005), “Biocatalysts and Enzyme Technology”, Wiley-VCH.
 H.W. Blanch & D.S. Clark (1996) "Biochemical Engineering", Marcel Dekker, Inc., N.Y
 G. Durand et P. Monsan (1982), "Les Enzymes - Production et Utilizations industrielles", Gauthier-Villars, Paris.
 J.E. Bailey & D.F.Ollis (1977), "Biochemical Engineering Fundamentals", McGrawHill Book CO, N.Y. (Chapters 4 and 5)
 D.I.C. Wang et al. (1979), "Fermentation and Enzyme Technology", John Wiley & Sons, N.Y..
Assessment for this course is through:
One unseen written examination, based on lecture material and practical classes (70%);
Practical classes (total of 30%), including the presentation and discussion of scientific papers, laboratorial classes, reports/essays and discussion.