Biosensors
Code
5324
Academic unit
Faculdade de Ciências e Tecnologia
Department
Departamento de Ciências dos Materiais
Credits
6.0
Teacher in charge
Hugo Manuel Brito Águas, Rui Alberto Garção Barreira do Nascimento Igreja
Weekly hours
5
Teaching language
Português
Objectives
To make available a broad revision of concepts behind the design and fabrication of biosensors. It is an objective of this course to introduce the students to the new sensoric technologies associated to biotechnology and microelectronics.
In the end of the course they should be able to:
1. Understand the physical, chemical and biological variables capable of being monitorized in a biological process.
2. Identify the various transduction systems available.
3. Should be able to master the main processes involved in the microfabrication
4. To propose a sensorial system capable of detecting a biological agent by means of physical, chemical and biological means involving Microelectronics.
Prerequisites
Basic formation in chemistry and physics areas.
Students with the formation given in the first years of the courses of Materials; Nanotechnology; Physics; Chemistry; Biochemistry; Biotechnology; Biomedics and similar areas
Subject matter
Introduction to biosensors. Bioreceptors and bioafinity. Principles and system of transduction. Physical properties of biological media. Temperature, pressure, force and displacement. Piezoelectric transduction systems. The piezoelectric cantilever. Microelectronic instruments. Optical transduction systems: fiber optics, optodes, evanescent waves, Surface Plasmon Resonance.
Electrochemical transduction systems: amperometric and potenciometric sensors. Surface immobilization. Supports for immobilization. Sol-gel, membranes, silica and polymeric supports. Calorimetric sensors. Immuno essays and immunosesnors. Enzimatic sensors. Micro-organisms based sensors. DNA sensors. Develpment of a immunosensor to detect IgG. ELISA, surface immobilization, immunosensor.
Instrumentation and data processing. Building of prototypes. Interdigital electrodes and electronic-nose.
Microfabrication. Microfabricated systems. Integrated systems. MEMS. Lab-in-a-chip. Instruments for the human health. rapid tests. Instruments for the applictions in biotechnology. Instruments for the monitoring of the environment. Biochips. Nanotechnology.
Bibliography
Handbook of Biosensors and Biochips, 2 Volume Set, Robert S. Marks (Editor), Christopher R. Lowe (Editor), David C. Cullen (Editor), Howard H. Weetall (Editor), Isao Karube (Editor), (2008) Wiley
Handbook of Modern Sensors: Physics, Designs, and Applications, Jacob Fraden, 3rd ed, (2004) Springer
Sensor Technology Handbook, Jon S. Wilson (Editor), (2005) Elsevier
Biosensors (Practical Approach S.) Jon Cooper, Tony Cass, 2nd Ed. (2004) Oxford University Press
John L. Vossen, Werner Kern, Thin Film Process II, Academic Press, 1991.
Cantilever transducers as a platform for chemical and biological sensors; Review of Scientific Instruments, Vol 75, nº 7, (2004)
BioMEMS: state-of-the-art in detection, opportunities and prospects; Rashid Bashir; Advanced Drug Delivery Reviews 56 (2004) 1565– 1586; (online na sciencedirect)
Microfabrication Techniques for Chemical/ Biosensors ; Proceedings of the IEEE, Vol. 91, nº 6, (2003)