Molecular Biology B
Code
1116
Academic unit
Faculdade de Ciências e Tecnologia
Department
Departamento de Ciências da Vida
Credits
6.0
Teacher in charge
Ilda Maria Barros dos Santos Gomes Sanches
Weekly hours
4
Total hours
65
Teaching language
Português
Objectives
The general objective of the course is to provide a comprehensive overview of the key concepts in molecular biology and uses of methods and techniques in molecular biology. Students are expected to acquire basic knowledge in molecular biology, and training in common methods to isolate and analyze DNA.
Prerequisites
none
Subject matter
The general objective of the course is to provide a comprehensive overview of the key concepts in molecular biology and uses of methods and techniques in molecular biology. Students are expected to acquire basic knowledge in molecular biology, and training in common methods to isolate and analyze DNA.
Laboratory classes: Growth and culturing of bacteria in liquid media. Preparation of serial dilutions for count colonies and determination of colony-forming units. Isolation, purification, and quantification of nucleic acids: plasmid DNA by alkaline lysis and cheek cell DNA extraction (adapted ‘DNA Extraction Module Genes in a Bottle” lab kit. Bio-Rad Lab.). UV spectrophotometry. Enzymatic hydrolysis of plasmid DNA. Agarose gel ectrophoresis to visualize isomeric forms of circular DNA molecules and DNA integrity. Physical mapping. PCR application in the detection of human Tas2R38 gene polymorphisms (phenotype associated with sensitivity to the bitter) in DNA samples from cells of the oral mucosa; restriction of the amplified DNA (PCR-RFLP) and analysis of T / NT (taster / non-taster) polymorphisms) of the Tas2R38 gene.
Bibliography
The following books contain chapters on content covered in classes:
Benjamin Lewin (2007). Genes IX. Jones and Bartlet Publishers.
http://biology.jbpub.com/book/genes/
Bruce Alberts, Alexander Johnson, Julian Lewis, Martin Raff, Keith Roberts, Peter Walter (2008. Molecular Biology of the Cell. Garland Science
http://www.scibooksdirect.com/covers/Misc_docs_1161312.pdf
Harvey Lodish; Arnold Berk, Chris A. Kaiser, Monty Krieger, Anthony Bretscher, Hidde Ploegh, Angelika Amon, Matthew P. Scott (2012). Molecular Cell Biology. W.H. Freeman
http://whfreeman.com/Catalog/product/molecularcellbiology-seventhedition-lodish
Sambrook J., and D. W. Russell. (2001) Molecular Cloning - A Laboratory Manual. 3rd Edition. Cold Spring Harbor Laboratory Press. New YorK
Teaching method
Lectures, laboratory and theorical-pratical sessions with quizzes